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Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 856

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 857

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 437

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 437

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 584

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 642

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 646

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 642

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 646

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 670

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 670

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 688

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 692

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 688

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 692

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 670

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 670

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 584

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 240

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 241

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 240

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 241

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 948

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 856

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 857

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 584

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 584

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 605

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 642

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 646

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 642

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 646

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 642

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 646

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 642

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 646

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 642

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 646

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 642

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 646

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 642

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 646

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 642

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 646

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 642

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 646

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 642

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 646

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 642

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 646

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 642

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 646

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 670

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 670

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 670

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 670

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 670

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 670

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 670

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 670

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 670

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 670

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 670

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 670

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 688

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 692

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 688

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 692

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 688

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 692

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 688

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 692

Deprecated: assert(): Calling assert() with a string argument is deprecated in /datas/vol3/w4a157096/var/www/wiki.nicelab.eu/htdocs/includes/diff/DairikiDiff.php on line 688

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(→‎Mechanical parts)
m (+ catégorie)
 
(9 révisions intermédiaires par un autre utilisateur non affichées)
Ligne 1 : Ligne 1 :
−
=== Open Bioreactor ===
+
[[Catégorie:Projets]]
 +
 
 +
== Open Bioreactor ==  
 +
''June 2013''
  
 
==== A polyvalent bioreactor ====
 
==== A polyvalent bioreactor ====
Ligne 38 : Ligne 41 :
 
* a windscreen washer's pump (optional)
 
* a windscreen washer's pump (optional)
  
−
<br><br><br><br>
+
<br><br><br><br><br><br>
  
 
====Openscad====
 
====Openscad====
Ligne 188 : Ligne 191 :
 
   );
 
   );
 
}
 
}
−
 
 
</nowiki>
 
</nowiki>
  
Ligne 202 : Ligne 204 :
 
The biohackerspace "La paillasse" is working on a similar project, I invite you to see:
 
The biohackerspace "La paillasse" is working on a similar project, I invite you to see:
 
http://www.lapaillasse.org/news/475/nom-de-code-bioreacteur/
 
http://www.lapaillasse.org/news/475/nom-de-code-bioreacteur/
 +
 +
''June 2013''

Version actuelle en date du 22 novembre 2013 à 14:53


Open Bioreactor

June 2013

A polyvalent bioreactor

"A bioreactor [...] refer to a device or system meant to grow cells or tissues in the context of cell culture. These devices are being developed for use in tissue engineering or biochemical engineering." wikipedia
the proposed design is a plug&play unique diver's, low cost and will insure those functions:

  • Soft brewing of cells, sucked up by air reflux, no helix requiered.
  • Oxygenation and CO2 control via Arduino
  • Photosynthesis
  • Controlled heating


His all in one design allows raw material economy, contaminant resistant by the lack of mobile jointure and adaptable to many jar. I/O signals are treated by an Arduino.

Initial volume 'll be 1 or 2 liters. More would be costly, and in accordance to many literature it's the standard lab's volume. By the way, by renewing the nutritive solution, a production mode is possible. An outside PHmeter could check the acidity of the solution by taking sample via a peristaltic pump.

It would be easy to build. All parts are easy to find and cheap. A printable version exists. For the emerging countries thoughts on models based on recycled raw materials must be done.

Mechanical parts

For the first prototype the following elements should be necessary:

  • 2 plastic pipes ∅22 et ∅35
    Openbioreacteur.jpg
    OpenBioreactor v2.png
  • Crystal tube, about 2 meters
  • a fish tank pump
  • a no return valve
  • HEPA filters from a vaccum
  • a tupperware box
  • an arduino
  • heat resistant epoxy glue
  • a thermoresistor CTN type
  • a computer's power suplly
  • T5 neon
  • a 7w lightbulb starter
  • heating wire or halogen lamp
  • 4 mosfets and 4 schotty diodes
  • a 1 or 2 liter jar
  • a windscreen washer's pump (optional)







Openscad

difference() {	
	cylinder(h=200,r=11,center=true);
	cylinder (h=240,r=10,center=true);		}

difference() {
	union(){cube([2,21,160],center=true);
		rotate(120,0) cube([2,21,160],center=true);
		rotate(60,0) cube([2,21,160],center=true);}
	cylinder(h = 200, r = 8.1, center = true);
}

difference(){
 	difference() {
		translate([0,0,40]) cylinder(h= 100,r= 15);
 		translate([0,0,38]) cylinder(h= 110,r= 14);}
	translate([0,-5,60]) rotate([90,90,0]) cylinder(h=15,r =1.5);
}

translate([-3,10,40]) cube([6,4,40]);

difference() {
	translate([-3,-14,40]) cube([6,4,40]);
	translate([0,-5,60]) rotate([90,90,0]) cylinder(h=15,r =1);
}

difference() {
	translate ([0,0,90]) cylinder(h=27,r=11.1);
	translate ([0,0,89.9]) cylinder(h=27.2,r=7.5);}

difference() {
translate([0,0,-88]) cylinder(h=5,r=16,center=true);
translate ([0,0,-80]) cylinder (h=30,r=10,center=true);		}

difference() {
translate ([0,0,-100]) metric_thread(26, 2, 12);
translate ([0,0,-95]) cylinder (h=20,r=10,center=true);		}

pi = 3.14159265;

function segments(diameter) = min(50, ceil(diameter*6));

module metric_thread(diameter=8, pitch=1, length=1, internal=false, n_starts=1)
{
 
   n_turns = floor(length/pitch);
   n_segments = segments(diameter);
   h = pitch * cos(30);

   union() {
      intersection() {
         for (i=[-1*n_starts : n_turns-1]) {
            translate([0, 0, i*pitch]) {
               metric_thread_turn(diameter, pitch, internal, n_starts);
            }
         }

         translate([0, 0, length/2]) {
            cube([diameter*1.1, diameter*1.1, length], center=true);
         }
      }

      if (internal) {
         cylinder(r=diameter/2 - h*5/8, h=length, $fn=n_segments);
      } else {

         cylinder(r=diameter/2 - h*5.3/8, h=length, $fn=n_segments);
      }
   }
}

module english_thread(diameter=0.25, threads_per_inch=20, length=1,
                      internal=false, n_starts=1)
{
   mm_diameter = diameter*25.4;
   mm_pitch = (1.0/threads_per_inch)*25.4;
   mm_length = length*25.4;

   echo(str("mm_diameter: ", mm_diameter));
   echo(str("mm_pitch: ", mm_pitch));
   echo(str("mm_length: ", mm_length));
   metric_thread(mm_diameter, mm_pitch, mm_length, internal, n_starts);
}

module metric_thread_turn(diameter, pitch, internal, n_starts)
{
   n_segments = segments(diameter);
   fraction_circle = 1.0/n_segments;
   for (i=[0 : n_segments-1]) {
      rotate([0, 0, i*360*fraction_circle]) {
         translate([0, 0, i*n_starts*pitch*fraction_circle]) {
            thread_polyhedron(diameter/2, pitch, internal, n_starts);
         }
      }
   }
}

function z_fct(current_radius, radius, pitch)
   = 0.5*(current_radius - (radius - 0.875*pitch*cos(30)))
                                                       /cos(30);

module thread_polyhedron(radius, pitch, internal, n_starts)
{
   n_segments = segments(radius*2);
   fraction_circle = 1.0/n_segments;

   h = pitch * cos(30);
   outer_r = radius + (internal ? h/20 : 0); 

   inner_r = radius - 0.875*h; 

   x_incr_outer = outer_r * fraction_circle * 2 * pi * 1.005;
   x_incr_inner = inner_r * fraction_circle * 2 * pi * 1.005;
   z_incr = n_starts * pitch * fraction_circle * 1.005;
   x1_outer = outer_r * fraction_circle * 2 * pi;
   z0_outer = z_fct(outer_r, radius, pitch);  
   z1_outer = z0_outer + z_incr;
   polyhedron(
      points = [
                [-x_incr_inner/2, -inner_r, 0],                                    
                [x_incr_inner/2, -inner_r, z_incr],                    
                [x_incr_inner/2, -inner_r, pitch + z_incr],           
                [-x_incr_inner/2, -inner_r, pitch],                               
                [-x_incr_outer/2, -outer_r, z0_outer],                             
                [x_incr_outer/2, -outer_r, z0_outer + z_incr],         
                [x_incr_outer/2, -outer_r, pitch - z0_outer + z_incr], 
                [-x_incr_outer/2, -outer_r, pitch - z0_outer]                     
               ],

      triangles = [
                [0, 3, 4],  
                [3, 7, 4], 
                [1, 5, 2],  
                [2, 5, 6],  
                [0, 1, 2],  
                [0, 2, 3],  
                [4, 6, 5],  
                [4, 7, 6],  
                [7, 2, 6], 
                [7, 3, 2], 
                [0, 5, 1],  
                [0, 4, 5]   
               ]
   );
}

prototype

Prototype open bioreacteur.jpg

Electronic

Coming soon

Links

The biohackerspace "La paillasse" is working on a similar project, I invite you to see: http://www.lapaillasse.org/news/475/nom-de-code-bioreacteur/

June 2013

Récupérée de « https://wiki.nicelab.eu/index.php?title=Open_bioreactor&oldid=5078 »